User Guide
Practical instructions for configuring a plate, running analysis, reading results, and exporting reports in HiPurA® Avian ELISAcheck software.
Getting Started
Use this checklist to complete one analysis run from start to export.
Open Analysis Page
Go to New Analysis from the sidebar or from the Home page.
Assign Well Roles
Select one or more wells on the 96-well grid, then assign a role: Blank, Positive Control (PC), Negative Control (NC), or Unknown (sample). You can click and drag to select multiple wells at once.
Enter Absorbance Data
Type OD values manually into the grid, or use the Import button to load data from a CSV or Excel (.xlsx) file exported by your plate reader.
Set Dilution Factors (Optional)
Switch to the Dilution tab to assign dilution factors to sample wells. Use presets (1x, 10x, 100x, 1000x), enter a custom value, or generate a serial dilution series. Default is 1x for all wells.
Run Analysis
Click Analysis, fill required metadata, and submit. The software computes the result and opens the Results page.
Plate Configuration
The 96-well plate grid is the core of the application. Here’s how to use it effectively.
Well Selection
Click individual wells or click and drag to select a range. Selected wells are highlighted on the grid.
Role Assignment
Use role controls to assign each selected well. Colors help verify the layout quickly.
Sample Naming
When assigning the Unknown role, you’ll be prompted to enter a sample name (e.g., S001). Samples with the same name are grouped in results.
Absorbance Tab
Enter or import raw OD (optical density) readings. Values above 4.0 are flagged as potentially invalid.
Dilution Tab
Record any additional dilutions made to the sample here, apart from those recommended in the protocol. For example, if the IFU requires a 1:100 dilution before the ELISA protocol, the dilution factor should be kept at 1X. If the user further dilutes the samples 5 times beyond the original 1:100 dilution (or dilutes directly to 1:500), the dilution factor should be recorded as 5X.
To apply the dilution factor, set the dilution factors per well. Choose from presets, enter custom values, or generate serial dilution series across rows or columns.
Import Data
Import CSV or Excel files from Thermo or Biotek plate readers. The format is auto-detected and values are mapped to the grid.
Understanding Results
After running an analysis, the results page shows calculated values for each well.
The original optical density value as read from the plate reader, without any adjustments.
Calculated as (Raw OD × Dilution Factor) − Mean Blank OD. Applies dilution correction and removes background noise.
Sample-to-Positive ratio. Calculated as Corrected OD of sample divided by mean Corrected OD of Positive Controls.
Percent Positivity — the S/P ratio expressed as a percentage. This is the primary value used to determine sample status.
Saving & Managing Layouts
Save your plate configurations for reuse across multiple analyses.
Save a Layout
Click Save Layout in the top-right corner of the Plate Configuration page. Enter a descriptive name and confirm.
Load a Layout
Click any saved layout from the Saved Layouts panel on the right to restore it. This overwrites the current plate configuration.
Rename or Delete
Use the edit icon to rename a layout or the delete icon to remove it permanently.
Export to File
Click the download icon to export a layout as a JSON file. This file contains well roles, OD values, and sample names.
Import from File
Click Import Layout to load a previously exported JSON layout file.
Reports & Export
After analysis, export reports directly from the Results page.
PDF Report
A complete, print-ready report including:
- Operator and organization details
- Run metadata and validation summary
- Full results table with color-coded status
- Validation criteria and test status
- Summary statistics (positive, negative, doubtful counts)
Excel Spreadsheet
An Excel (.xlsx) file with:
- All sample data in a structured table format
- Well ID, Sample ID, Raw OD, Dilution, Corrected OD, PP%, and Status
- Easy to import into other analysis tools or LIMS systems
Tips & Shortcuts
Multi-Well Selection
Click and drag across the plate grid to select multiple wells at once. Assign roles to all selected wells in a single action.
Wavelength Configuration
The default wavelength is 450 nm. You can change it in the status bar at the bottom of the Plate Configuration page.
Use Representative Test Data
Validate your layout and thresholds on representative data before processing critical batches.
Serial Dilution
In the Dilution tab, use the serial dilution generator to automatically fill a range of wells with increasing dilution factors.
Validation Check
Ensure Blank, Positive Control, and Negative Control wells are assigned before running analysis. The software validates PC OD meets the minimum threshold.
Current Session Storage
The app is configured without database persistence. Export reports/files when you need permanent records.